Rapid Pulsed-Field Gel Electrophoresis Method for Group B Streptococcus Isolates
نویسندگان
چکیده
منابع مشابه
Rapid pulsed-field gel electrophoresis method for group B streptococcus isolates.
We developed a rapid pulsed-field gel electrophoresis (PFGE) method that required 3 days to complete, an improvement over the standard method that required as many as 8 days. The accuracy and reproducibility of the rapid method were verified by analysis of DNA band sizes of our control group B streptococcus isolate. The rapid method was superior to the standard method, providing more precise mo...
متن کاملPulsed field gel electrophoresis analysis conditions for molecular epidemiological group B streptococcus identification.
Hiroko WAKIMOTO1)2), Masamichi OGURA2), Hisako YANO2), Akira KOGUCHI3), Yukio WAKIMOTO4), Souhei MIYAKAWA5), Matsuko Katsuko OKUZUMI6) & Masashi MIZOKAMI4) 1) Aichi Medical University School of Nursing 2) Nagoya City University Graduate School of Nursing 3) Abbott Japan 4) Nagoya City University Hospital 5) Kariwa-gun General Hospital 6) Dokkyo Medical University Hospital (Received November 2, ...
متن کاملPulsed-field gel electrophoresis.
DNA molecules in an agarose matrix elongate and migrate toward the anode when exposed to an electric field. An agarose matrix represents a highly irregular network with pores of various dimensions, large open areas, and regions with different densities. During electrophoresis, migration will be retarded by obstructions that prevent sieving by size and result in "end-on" migration as if through ...
متن کاملCharacterization of Streptococcus pneumoniae ophthalmic, systemic & commensal isolates by pulsed-field gel electrophoresis & ribotyping.
BACKGROUND & OBJECTIVE Streptococcus pneumoniae is common in ocular and systemic infections and is a part of normal nasopharyngeal flora. Very few studies regarding genetic analysis of S. pneumoniae isolates causing eye infections are available. This study was undertaken to do pulse field gel electrophoresis (PFGE) analysis and ribotyping of S. pneumoniae isolates obtained from eye infections, ...
متن کاملMethod for optimizing pulsed-field gel electrophoresis banding pattern data.
The genomic DNA of 47 strains of TSST-1 toxin-producing Staphylococcus aureus were cleaved with SmaI restriction endonuclease and resolved in an agarose gel by pulsed-field gel electrophoresis (PFGE). An algorithm was designed to standardize the band weights or brightness (trace quantity) produced to a bounded region between 0 and 1 regardless of DNA fragment size while simultaneously reducing ...
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ژورنال
عنوان ژورنال: Journal of Clinical Microbiology
سال: 2001
ISSN: 0095-1137,1098-660X
DOI: 10.1128/jcm.39.8.3006-3008.2001